Last updated on July 23, 2015
Due to a failed instrument (camera) and very high usage, we are running about 36 hours behind, so you data will likely be delayed. Hopefully we will be caught up by Monday, 7/27.
Operation:Users provide DNA (plasmid, phage or PCR product) at a standardized concentration and custom primers if necessary, and the Facility performs cycle sequencing reactions using fluorescent dye terminators, runs the capillaries, acquires the data, and provides the sequence as chromatogram and text files. We also run user's reactions, and genotyping (fragment analysis) samples. For chromatogram editing, software is available for Macintosh and Windows platforms (see below). The current charge is $4.25 (Univ. of Chicago) or $7.00 (external) per reaction of a single template and primer ($2.25 and $3 [internal] and $4 [external] to run user reactions). Users are guaranteed satisfactory sequence from their orders even if it requires resequencing new template DNA (there is a 24 reaction limit), or the failed reactions are free. Click here for our redo policy.We are committed to giving every user satisfactory sequence.
Our turnaround time is 1 business day from receipt of samples (multi-plate orders may be slightly longer).
For high-throughput sequencing ($1.70 to $2.70/rxn, 24 reactions minimum) or genotyping, custom services, such as complete sequencing projects, primer design and synthesis, etc, please call us at (773)702-1088.
Submitting Sequencing, Genotyping and Colony Picking Orders:
For Retrieving Finished Sequences and Interpreting Chromatograms:
Software for Viewing and Editing Chromatograms (required!):
For further information please contact us by phone at (773) 702-1088 or e-mail.