We support standard genotyping with Applied Biosystems dye sets D (4-dye set: 6FAM, HEX, NED, with ROX 500 as standard) and G5 (5 dye set: 6FAM, VIC, NED, PET, with LIZ 500 as standard), as well as SnapShot and SNPlex.
Please give samples reasonably short names, avoiding names longer than 23 characters or names with spaces, quotes, or slashes present. Organize samples in the plate to avoid gaps where possible. Samples MUST be organized in the 8-well dimension in plates, with samples running from 01A, 01B, 01C, etc.
Please use the following Excel spreadsheet (zipped for downloading) to place your order for standard genotyping and SnapShot: Genotyping_template.
Use the following spreadsheets (zipped for downloading) for SNPlex reactions, being sure to fill in the colored cells (96-well plates): SNPlex96_template.
or 384-well plates): SNPlex384_template.
Please e-mail the file to us at DNACore@DNAServer.bsd.uchicago.edu as an attachment, changing the name of the file to include your name and the date (eg. J-Doe_8-1-06.xls).
For analyzing your data, we have a copy of GeneMapper 4.0 on a user-accessible computer in the facility. Otherwise for simple sizing analyses, you can download a copy of Peak Scanner from the appliedbiosystems.com web site (Windows only). Third party software is also available (eg. Geospiza.com).