Terrific Broth (TB)

quantity/liter

Tryptone

12 g

Yeast Extract

24 g

glycerol

4 ml

KH2PO4

2.31 g

K2HPO4

12.54 g

 

LB-M9

g/l

Na2HPO4 (141.96 MW)

7.0

KH2PO4 (136.1 MW)

2.0

NaCl

0.5

NH4Cl

1.0

Glucose

2.0

sodium succinate

1.0

Tryptone

10.0

Yeast extract

5.0

(optional after autoclaving)

2 ml 1 M MgSO4

0.1 ml 1 M CaCl2